2018-02-13T16:06:05.000Z2025-04-04 06:46:59

locus

BSU14580

BSU_14580

geneLength

1113

1116

outlinks

bsu

BSU14580

BSU_14580

Gene

Coordinates

1,528,326 → 1,529,441

1,528,326 1,529,441

The protein

Catalyzed reaction/ biological activity

Pyruvate [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine CO₂ (according to Swiss-Prot)

[dihydrolipoyllysine-residue acetyltransferase]-(R)-N⁶-lipoyl-L-lysine + H⁺ + pyruvate --> [dihydrolipoyllysine-residue acetyltransferase]-(R)-N⁶-(S⁸-acetyldihydrolipoyl)-L-lysine + CO₂ (according to UniProt)

The protein

Paralogous protein(s)

[[protein|AcoA]], [[protein|BkdAA]]

[[this]]

Biological materials

Mutant

BKE14580 (Δ[[gene|pdhA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA

BKK14580 (Δ[[gene|pdhA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA

BKE14580 ([[gene|pdhA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA

BKK14580 ([[gene|pdhA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTAAGTCACCTCTTCC, downstream forward: _UP4_ACACAGAAGGAGTCGAAGTA

Biological materials

lacZ fusion

pGP721 (in [[protein|pAC5]]), available in [SW|Stülke] lab, pGP186 (in [[protein|pAC7]]), available in [SW|Stülke] lab

pGP721 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab, pGP186 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]

References

Original publications

9352926, 20525796, 12850135, 6414463, 11976308, 20081037, 15378759, 24825009, 28189581, 28516784

9352926, 20525796, 12850135, 6414463, 11976308, 20081037, 15378759, 24825009, 28189581, 28516784, 30782632

Expression and Regulation

additional information

the mRNA belongs to the 46 most abundant mRNA species in dormant spores [pubmed|30782632]

labs

[SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]